Generator

Part:BBa_K346085:Design

Designed by: Junyi Jiao   Group: iGEM10_Peking   (2010-10-18)

Ptet-RBS-T7polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Just ligate the promoter, RBS and T7polymerase together by subclone to get the generator.



Source

It is necessary to point that Ptet is a constitutive promoter only in the trans5alpha strain.


References